Shyamala G, Leonard L
J Biol Chem. 1980 Jul 10;255(13):6028-31.
The effects of sodium molybdate on the transformation of mouse uterine cytoplasmic estradiol . receptor complex has been studied. If molybdate is added to cytosol containing the nontransformed estradiol . receptor complex, it blocks the receptor transformation which otherwise occurs during incubation at 28 degrees C. The inhibition by molybdate is apparent both in sucrose gradient centrifugation analyses of estradiol . receptor complexes and [3H]estradiol dissociation kinetic studies. A molybdate-sensitive change of the nontransformed estradiol . receptor complex can also be inferred from behavior of the estradiol . receptor complex on low ionic strength sucrose gradients.
已对钼酸钠对小鼠子宫细胞质雌二醇受体复合物转化的影响进行了研究。如果将钼酸盐添加到含有未转化雌二醇受体复合物的胞质溶胶中，它会阻断在28℃孵育期间原本会发生的受体转化。钼酸盐的抑制作用在雌二醇受体复合物的蔗糖梯度离心分析和[3H]雌二醇解离动力学研究中均很明显。未转化雌二醇受体复合物的钼酸盐敏感性变化也可从雌二醇受体复合物在低离子强度蔗糖梯度上的行为推断出来。
