Markus R P, Lapa A J, Valle J R
J Pharmacol Exp Ther. 1980 Aug;214(2):423-6.
Castration of the guinea pig was followed by atrophy of the vas deferens and appearance of spontaneous contractions. Testosterone replacement reversed those effects and diethylstilbestrol treatment increased them. A month after castration or castration and stilbestrol treatment (15 mg/30 days), the membrane exhibited depolarization and spontaneous spikes that were not seen with the normal groups. The spontaneous contractions observed with both groups reflected the occurrence of membrane spikes. Tetrodotoxin (3 c 10(-6) M) did not change the membrane potential in any organ studied nor did it affect the pattern of spontaneous contractions. Verapamil (5 x 10(-6) M) increased the membrane potential of the muscles from castrated and stilbestrol-treated castrated animals, abolished the membrane spikes and blocked the spontaneous contractions. The results suggest that castration increases calcium permeability through the membrane. The increase in calcium permeability would be responsible for muscle rhythmicity.
豚鼠去势后，输精管萎缩并出现自发收缩。睾酮替代可逆转这些效应，而己烯雌酚治疗则增强这些效应。去势或去势并己烯雌酚治疗（15毫克/30天）一个月后，细胞膜出现去极化和正常组未见的自发尖峰。两组观察到的自发收缩反映了膜尖峰的出现。河豚毒素（3×10⁻⁶摩尔）对所研究的任何器官的膜电位均无影响，也不影响自发收缩模式。维拉帕米（5×10⁻⁶摩尔）增加了去势及己烯雌酚治疗的去势动物肌肉的膜电位，消除了膜尖峰并阻断了自发收缩。结果表明，去势增加了细胞膜对钙的通透性。钙通透性的增加可能是肌肉节律性的原因。
