Il'ina T K, Negru-Vodé V V, Miller Iu M, Kapustin O A, Khodakova R N
Mikrobiologiia. 1980 Mar-Apr;49(2):284-7.
The energy process of nitrate reduction was studied in the typical soil denitrifying cultures of Pseudomonas denitrificans by mass spectrometry. These microorganisms differed in certain characteristics of the process from the cultures of sporogenic soil denitrifying bacteria belonging to the Bacillus genus. The rate of denitrification by Ps. denitrificans was very high; as the result, losses of 15N from the medium exceeded 80--90%. Ammonia was not produced as the final product of nitrate reduction in the course of denitrification. Apparently, hydroxylamine, hyponitrite or an organic intermediate product of nitrate reduction is produced in the process of its assimilation by the cultures.
采用质谱分析法对反硝化假单胞菌典型土壤反硝化培养物中的硝酸盐还原能量过程进行了研究。这些微生物在该过程的某些特性上与芽孢杆菌属的产孢土壤反硝化细菌培养物有所不同。反硝化假单胞菌的反硝化速率非常高;结果,培养基中15N的损失超过了80%-90%。在反硝化过程中,硝酸盐还原的最终产物不是氨。显然,在培养物对硝酸盐的同化过程中会产生羟胺、连二次硝酸或硝酸盐还原的有机中间产物。