Culp L A
Nature. 1980 Jul 3;286(5768):77-9. doi: 10.1038/286077a0.
Study of the biological function of the cells of the nervous system has benefited from the isolation of cloned glial or neuronal tumour cell lines that can be induced to differentiate in culture. Many of their differentiated properties reflect specific biochemical changes at the cell surface, and we have observed changes in cytoskeletal organization and glycosaminoglycan-containing proteoglycan content in the tissue culture substratum adhesion sites of neuroblastoma cells during neurite extension. To assist in defining structure function relationships of cell surface components of rodent neuroblastoma cells, I have used two cell-surface-active agents to select for phenotypically-stable varinats of these cells which display some unique properties and which will be useful for further biochemical and physiological analyses.
对神经系统细胞生物学功能的研究受益于克隆的神经胶质或神经元肿瘤细胞系的分离，这些细胞系在培养中可被诱导分化。它们的许多分化特性反映了细胞表面特定的生化变化，并且我们观察到在神经母细胞瘤细胞的神经突延伸过程中，其组织培养基质粘附位点的细胞骨架组织和含糖胺聚糖的蛋白聚糖含量发生了变化。为了帮助确定啮齿动物神经母细胞瘤细胞表面成分的结构功能关系，我使用了两种细胞表面活性剂来选择这些细胞的表型稳定变体，这些变体具有一些独特的特性，将有助于进一步的生化和生理分析。
