Kumar S, Rathi M
Neurochem Res. 1980 May;5(5):547-50. doi: 10.1007/BF00964991.
A procedure for the purification of guanine deaminase inhibitor from human brain mitochondria is described. The inhibitor was enriched about 150-fold with recoveries of over 65%. It is nondialyzable, insoluble in water, and stable for over 30 days at -16 degrees C. However, the protein is completely inactivated at 50 degrees C in 5 min. The purified protein also inhibits the activities of a number of other enzymes.
描述了一种从人脑海马体中纯化鸟嘌呤脱氨酶抑制剂的方法。该抑制剂富集了约150倍，回收率超过65%。它不能透析，不溶于水，在-16℃下可稳定保存30多天。然而，该蛋白质在50℃下5分钟内会完全失活。纯化后的蛋白质还能抑制许多其他酶的活性。
