van der Mast C, Voorma H O
Biochim Biophys Acta. 1980 May 30;607(3):512-9. doi: 10.1016/0005-2787(80)90161-6.
The eukaryotic initiation factors eIF-4A and eIF-4D are almost exclusively found in the supernatant of a reticulocyte lysate. The basic steps of general purification schemes failed because these factors do not bind to Sepharoseheparin or, in the of eIF-4A, to phosphocellulose and new procedure had to be devised. Cibacron Blue F3G-A proved to be a successful alternative in the purification of these factors from the excessive load of proteins found in the supernatant.
真核生物起始因子eIF-4A和eIF-4D几乎只存在于网织红细胞裂解物的上清液中。常规纯化方案的基本步骤失败了，因为这些因子不与琼脂糖肝素结合，就eIF-4A而言，也不与磷酸纤维素结合，因此必须设计新的方法。事实证明，在从上清液中大量蛋白质中纯化这些因子时，汽巴蓝F3G-A是一种成功的替代方法。
