不均一核RNA分子量的发育变化

Developmental changes in the molecular weight of heterogeneous nuclear RNA.

作者信息

Dubroff L M

出版信息

Biochim Biophys Acta. 1980 Jul 29;608(2):378-86. doi: 10.1016/0005-2787(80)90183-5.

Abstract

The size distribution of heterogeneous nuclear RNA (hnRNA) in the sea urchin embryo changes markedly during early development. Measurement of cleavage (4.5 h) to late gastrula (40 h) hnRNA by sedimentation in aqueous and denaturing solvent indicates that in the stages tested cleavage (4.5 h) hnRNA is smallest and rotating blastula (13 h) hnRNA is largest. The molecular weight of cleavage hnRNA is calculated to be about one-third that of rotating blastula hnRNA. Sedimentation of early embryo hnRNA in denaturing solvent to disaggregate complexes demonstrated mean S values lower than those obtained in aqueous solvent.

摘要

海胆胚胎中异质性核RNA（hnRNA）的大小分布在早期发育过程中发生显著变化。通过在水性和变性溶剂中沉降来测量从卵裂期（4.5小时）到原肠胚后期（40小时）的hnRNA，结果表明在所测试的阶段中，卵裂期（4.5小时）的hnRNA最小，而旋转囊胚期（13小时）的hnRNA最大。卵裂期hnRNA的分子量经计算约为旋转囊胚期hnRNA的三分之一。早期胚胎hnRNA在变性溶剂中沉降以解离复合物，其平均S值低于在水性溶剂中获得的S值。