碘氨基酸和碘化蛋白质的差示分光光度法。碘酪氨酸、甲状腺素和甲状腺球蛋白的乙二醇水溶液。
Differential spectrophotometry of iodoamino acids and iodinated proteins. Aqueous ethylene glycol solutions of iodotyrosines, thyroxine and thyroglobulin.
作者信息
Akhmedzhanov I G, Gussakovsky E E, Babaev T A
出版信息
Biochim Biophys Acta. 1980 Jun 26;623(2):381-8. doi: 10.1016/0005-2795(80)90267-6.
Solvent perturbation difference spectra and corresponding molar extinction coefficients of solutions of monoiodotyrosine, diiodotyrosine and thyroxine with protonated and deprotonated hydroxyls were measured with 20% ethylene glycol as perturbant. Data obtained were applicable to the development of the method of quantitative estimation of the number of iodotyrosine and thyroxine residues accessible to ethylene glycol in iodinated proteins. This method was used for the determination of iodoamino acids available to ethylene glycol in bovine thyroglobulin (naturally iodinated protein).
以20%的乙二醇作为扰动剂，测量了单碘酪氨酸、二碘酪氨酸和甲状腺素溶液在羟基质子化和去质子化状态下的溶剂扰动差光谱及相应的摩尔消光系数。所获得的数据适用于开发定量估算碘化蛋白质中可被乙二醇接触到的碘酪氨酸和甲状腺素残基数量的方法。该方法用于测定牛甲状腺球蛋白（天然碘化蛋白质）中可被乙二醇接触到的碘氨基酸。
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