Aubin M, Prud'homme R E, Pézolet M, Caillé J P
Biochim Biophys Acta. 1980 Aug 1;631(1):90-6. doi: 10.1016/0304-4165(80)90056-2.
Differential scanning calorimetry has been used to investigate the state of water in intact single muscle fibers of the giant barnacle, Balanus nubilus. The shapes of the melting curves suggest the presence of three types of water: unfrozen (or bound), free (or bulk) and intermediate water. The amount of unfrozen water per g protein was constant within experimental error. An increase in water content changed almost exclusively the amounts of free water. The amount of intermediate water varies only slightly with the fiber water content.
差示扫描量热法已被用于研究巨型藤壶（Balanus nubilus）完整单肌纤维中的水状态。熔融曲线的形状表明存在三种类型的水：未冻结（或结合）水、自由（或大量）水和中间水。在实验误差范围内，每克蛋白质中未冻结水的量是恒定的。含水量的增加几乎只改变了自由水的量。中间水的量仅随纤维含水量略有变化。
