Fillion G, Fillion M P
Eur J Pharmacol. 1980 Jul 11;65(1):109-12. doi: 10.1016/0014-2999(80)90218-6.
Binding of [3H]5-HT was studied using purified neuronal membranes isolated from horse brain striatum. It is shown that the preincubation of these membranes with an agonist (5-HT, bufotenin) followed by washing, modified the binding of [3H]5-HT from a low affinity to a high affinity constant. This effect was blocked by previous pretreatment of the membranes with a sulfhydryl group reagent (N-ethylmaleimide) and was reversed by guanoside 5'-triphosphate.
利用从马脑纹状体分离出的纯化神经元膜研究了[3H]5-羟色胺（5-HT）的结合情况。结果表明，这些膜与激动剂（5-HT、蟾蜍色胺）预孵育后洗涤，会使[3H]5-HT的结合从低亲和力常数转变为高亲和力常数。这种效应被膜预先用巯基试剂（N-乙基马来酰亚胺）处理所阻断，并且被鸟苷5'-三磷酸逆转。
