Entenmann A H, Sierralta W, Jungblut P W
Hoppe Seylers Z Physiol Chem. 1980;361(6):959-68. doi: 10.1515/bchm2.1980.361.1.959.
Endometrial cells from prepuberal pigs contain an estradiol 17 beta-dehydrogenase. By differential centrifugation of homogenate fractions and isopycnic separation of the particles sedimenting in the 600 leads to 38000 x g range, accompanied by marker enzyme and electron microscopic analyses, the enzyme appears to be exclusively associated with a subpopulation of lysosomes. It displays a high affinity for estradiol with a Km = 5 x 10(-7) mol/l. Dehydrogenation of estradiol to estrone is favorably driven by NAD, poorly by NADP. The reverse reaction with estrone as substrate and either NADH or NADPH or NADPH + regenerating system as cosubstrate does not proceed beyond the detection level, but trace amounts of 6 alpha/7 alpha-hydroxyestrone are observed at pH 6.5 with the latter two cosubstrates.
青春期前猪的子宫内膜细胞含有一种17β-雌二醇脱氢酶。通过对匀浆组分进行差速离心以及对在600至38000×g范围内沉降的颗粒进行等密度分离，并结合标记酶和电子显微镜分析，该酶似乎仅与溶酶体的一个亚群相关。它对雌二醇具有高亲和力，Km = 5×10(-7) mol/l。雌二醇脱氢生成雌酮的反应由NAD良好驱动，由NADP驱动较差。以雌酮为底物、以NADH或NADPH或NADPH + 再生系统为共底物的逆反应未超过检测水平，但在pH 6.5时，后两种共底物会观察到痕量的6α/7α-羟基雌酮。
