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Rat lens epithelial cells in vitro. II. Changes of protein patterns during aging and transformation.

作者信息

Rink H, Vornhagen R

出版信息

In Vitro. 1980 Apr;16(4):277-80. doi: 10.1007/BF02618332.

DOI:10.1007/BF02618332
PMID:7399543
Abstract

Serially subcultured rat lens epithelial cells grow in different stages, which can be classified according to morphology, chromosome numbers and population kinetics. A lens-specific gamma-crystallin appears in the diploid stage, when elongated cell types are observed. One of the beta-crystallin bands (pH 5.7) disappears during aging in higher passage numbers of the diploid stage B. A weak band in the beta-crystallin region (pH 6.4), which is present in all stages, becomes very intensive in aneuploid cells of stage D, which exhibit a fibroblast-like morphology.

摘要

相似文献

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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS.抗原-抗体交叉电泳
Anal Biochem. 1965 Feb;10:358-61. doi: 10.1016/0003-2697(65)90278-2.
3
Rat lens epithelial cells in vitro. I. Observations on aging, differentiation and culture alterations.体外培养的大鼠晶状体上皮细胞。I. 关于衰老、分化及培养变化的观察
In Vitro. 1980 Jan;16(1):15-9. doi: 10.1007/BF02618195.
4
The expression of differentiation by chicken lens epithelium in in vitro cell culture.
Dev Growth Differ. 1971 Dec;13(4):323-36. doi: 10.1111/j.1440-169x.1971.00323.x.
5
Isoelectric focusing of lens crystallins in thin-layer polyacrylamide gels. A method for detection of soluble proteins in eye lens extracts.晶状体蛋白在薄层聚丙烯酰胺凝胶中的等电聚焦。一种检测眼晶状体提取物中可溶性蛋白质的方法。
J Chromatogr. 1971 Aug 26;60(2):225-33. doi: 10.1016/s0021-9673(00)95554-7.
6
Characteristics of cultured lens epithelium.培养的晶状体上皮细胞的特征。
Invest Ophthalmol. 1969 Aug;8(4):393-400.
7
Molecular aspects of lens cell differentiation.
Science. 1967 Apr 21;156(3773):338-46. doi: 10.1126/science.156.3773.338.
8
Cultured calf lens epithelium. I. Methods of cultivation and characteristics of the cultures.培养的小牛晶状体上皮细胞。I. 培养方法及培养物特性
Exp Eye Res. 1974 Jun;18(6):517-26. doi: 10.1016/0014-4835(74)90058-x.
9
Isoelectric focusing of embryonic cow lens crystallins.胚胎牛晶状体晶状体蛋白的等电聚焦
Exp Eye Res. 1973 Jun;16(2):131-42. doi: 10.1016/0014-4835(73)90308-4.
10
Cell elongation in the cultured embryonic chick lens epithelium with and without protein synthesis. Involvement of microtubules.有或无蛋白质合成情况下培养的鸡胚晶状体上皮细胞的伸长。微管的作用。
J Cell Biol. 1972 Oct;55(1):82-92. doi: 10.1083/jcb.55.1.82.