Hepler P K
J Cell Biol. 1980 Aug;86(2):490-9. doi: 10.1083/jcb.86.2.490.
Membranes in the mitotic apparatus have been investigated ultrastructually in dividing cells of barley (Hordeum vulgare). After osmium tetroxide- potassium ferricyanide or ferrocyanide postfixation (OsFeCN) of material that had been fixed in glutaraldehyde in the presence of Ca(++), the nuclear envolope (NE)-endoplasmic reticulum (ER) complex is selectively stained, permitting observations on the cellular pattern and structural ramifications of this membrane system that have not been previously recognized. Specifically, it is observed that membrane system that have not been previously recognized. Specifically, it is observed that during mitosis the NE-ER forms a continuous membrane system that ensheathes and isolates the mitotic apparatus (MA). Elements of ER progressively accumulate in the region of the spindle pole, becoming most concentrated by early anaphase. Within the MA itself, there are striking spindle- membrane associations; in particular, tubular elements of predominantly smooth NE-ER invade the spindle interior selectively along kinetochore microtubules. The membrane elements at the pole and surrounding the MA consist of tubular reticulum and fenestrated lamellae. Membranes of the MA thus resemble in considerable detail the tubular network and fenestrated elements of the sarcoplasmic reticulum of muscle. It is suggested that the NE-ER of the dividing barley cell may function in one or both of the following ways: (a) to control the concentration of free Ca(++) in the MA and (b) to serve as an anchor to chromosome motion.
在大麦（Hordeum vulgare）的分裂细胞中，已经对有丝分裂器中的膜进行了超微结构研究。在用戊二醛在Ca(++)存在下固定的材料经四氧化锇 - 铁氰化钾或亚铁氰化钾后固定（OsFeCN）后，核膜（NE） - 内质网（ER）复合体被选择性染色，从而可以观察到这个膜系统以前未被认识的细胞模式和结构分支。具体而言，可以观察到以前未被认识的膜系统。具体来说，观察到在有丝分裂期间，NE - ER形成一个连续的膜系统，包裹并隔离有丝分裂器（MA）。内质网的成分在纺锤体极区域逐渐积累，到后期早期变得最为集中。在有丝分裂器内部，有明显的纺锤体 - 膜关联；特别是，主要是光滑的NE - ER的管状成分沿着动粒微管选择性地侵入纺锤体内部。极处和围绕有丝分裂器的膜成分由管状网状结构和有孔薄片组成。因此，有丝分裂器的膜在相当程度上类似于肌肉肌质网的管状网络和有孔成分。有人提出，分裂的大麦细胞的NE - ER可能以以下一种或两种方式发挥作用：（a）控制有丝分裂器中游离Ca(++)的浓度，（b）作为染色体运动的锚定物。
