在含钙浓度低的溶液中体外微管的形成。
Microtubule formation in vitro in solutions containing low calcium concentrations.
作者信息
Weisenberg R C
出版信息
Science. 1972 Sep 22;177(4054):1104-5. doi: 10.1126/science.177.4054.1104.
PMID:4626639
Abstract
Isolated rat brain tubulin can be repolymerized in vitro in solutions containing adenosine triphosphate or guanosine triphosphate, magnesium ions, and a good calcium chelator. The extreme sensitivity of tubulin to calcium ions explains the failure of previous efforts to obtain polymerization and suggests a possible mechanism for regulation of microtubule polymerization in vivo.
摘要
分离出的大鼠脑微管蛋白可在含有三磷酸腺苷或三磷酸鸟苷、镁离子和优质钙螯合剂的溶液中于体外重新聚合。微管蛋白对钙离子的极度敏感性解释了此前获得聚合反应的努力为何失败，并提示了一种体内微管聚合调控的可能机制。
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