Petersen M C, Nation R L, Ashley J J
J Chromatogr. 1980 Aug 8;183(2):131-9. doi: 10.1016/s0378-4347(00)81686-2.
A sensitive, specific, reproducible and rapid high-performance liquid chromatographic method for the simultaneous quantitation of betamethasone, betamethasone 21-acetate and hydrocortisone in biological fluids is described. Hydrocortisone acetate is used as an internal standard and the samples are extracted with dichloromethane before chromatographing on a reversed-phase system. Detection at two ultraviolet wavelengths (254 nm and 240 nm) was used to assess the specificity of the system, and the sensitivity was found to be greater than 10 ng for all steroids. The speed with which this assay can be performed makes it particularly useful for pharmacokinetic studies, and plasma concentration--time profiles resulting from the administration of betamethasone phosphate and betamethasone acetate are presented.
本文描述了一种灵敏、特异、可重现且快速的高效液相色谱法，用于同时定量生物流体中的倍他米松、倍他米松21 - 乙酸酯和氢化可的松。醋酸氢化可的松用作内标，样品在反相系统上进行色谱分析前用二氯甲烷萃取。采用在两个紫外波长（254 nm和240 nm）处检测来评估该系统的特异性，发现所有甾体的灵敏度均大于10 ng。该测定方法的执行速度使其对药代动力学研究特别有用，并给出了磷酸倍他米松和醋酸倍他米松给药后的血浆浓度 - 时间曲线。
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