Grundmann E, Petzinger E, Frimmer M, Boschek C B
Naunyn Schmiedebergs Arch Pharmacol. 1978 Dec;305(3):253-9. doi: 10.1007/BF00498819.
In contrast to normal liver cells, AS-30D hepatoma cells are insensitive to phalloidin. The lack of the typical phalloidin response in the latter cells is not due to a deficiency of contractile proteins. Actin prepared from hepatoma cells is able to form filamentous structures and is stabilized in a manner similar to muscle actin. Isolated liver cells were exposed to a medium containing phalloidin and removed after 20 min by centrifugation. The supernatant was incubated again with fresh cells. The procedure was repeated four times. The phalloidin response decreased to about 19% of the control because of the uptake of phalloidin during each incubation. When the same procedure was carried out with AS-30D hepatoma cells, and aliquots of the supernatants were tested with hepatocytes no marked decrease of the phalloidin response was seen. This indicates that hepatoma cells do not consume the toxin as do normal liver cells.
与正常肝细胞相比，AS - 30D肝癌细胞对鬼笔环肽不敏感。后者细胞缺乏典型的鬼笔环肽反应并非由于收缩蛋白的缺乏。从肝癌细胞制备的肌动蛋白能够形成丝状结构，并以类似于肌肉肌动蛋白的方式稳定下来。将分离的肝细胞暴露于含有鬼笔环肽的培养基中，20分钟后通过离心去除。将上清液再次与新鲜细胞一起孵育。该过程重复四次。由于每次孵育期间鬼笔环肽的摄取，鬼笔环肽反应降至对照的约19%。当对AS - 30D肝癌细胞进行相同的操作，并用上清液的等分试样检测肝细胞时，未观察到鬼笔环肽反应有明显下降。这表明肝癌细胞不像正常肝细胞那样消耗毒素。
