Dusek Z, Hradec J
Neoplasma. 1978;25(5):609-16.
Protein synthesis was significantly enhanced in subcellular systems containing ribosomes and cytosol from the liver of Walker tumor-bearing rats from the second week following the tumor transplantation and this enhancement persisted for the whole period of tumor growth. Homologous systems from Zajdela hepatoma and host liver showed a markedly increased poly(U)-dependent peptide elongation when compared with normal liver tissue. A stimulation of polyphenylalanine synthesis resulted from the addition of cytosols from tumors or host liver to ribosomes from normal rat liver. Similar results were found for the binding of phenylalanyl-tRNA to ribosomes. Ribosomes from tumors and host liver are more active in peptide elongation than particles from normal liver tissue. A more than 10-fold stimulation of phenylalanine polymerization resulted from the addition of poly(U) to ribosomes from Zajdela hepatoma whereas only less than 2-fold enhancement was found when using ribosomes from normal or host liver. Hepatoma ribosomes apparently contain only a low proportion of polyribosomes carrying natural message. Enhanced protein synthesis in tumors and host liver is apparently due, in particular, to an increased activity of soluble factors required for protein synthesis and less due to an increased activity of ribosomes.
从肿瘤移植后第二周起，含有Walker荷瘤大鼠肝脏核糖体和胞质溶胶的亚细胞系统中蛋白质合成显著增强，且这种增强在肿瘤生长的整个阶段持续存在。与正常肝组织相比，来自Zajdela肝癌和宿主肝脏的同源系统显示出聚（U）依赖性肽链延伸显著增加。将肿瘤或宿主肝脏的胞质溶胶添加到正常大鼠肝脏的核糖体中，可刺激聚苯丙氨酸的合成。苯丙氨酰 - tRNA与核糖体的结合也得到类似结果。肿瘤和宿主肝脏的核糖体在肽链延伸方面比正常肝组织的颗粒更活跃。向Zajdela肝癌的核糖体中添加聚（U）可使苯丙氨酸聚合受到超过10倍的刺激，而使用正常或宿主肝脏的核糖体时，仅发现不到2倍的增强。肝癌核糖体显然仅含有低比例携带天然信息的多核糖体。肿瘤和宿主肝脏中蛋白质合成的增强显然尤其归因于蛋白质合成所需可溶性因子活性的增加，而较少归因于核糖体活性的增加。
