Oxford J S, Corcoran T, Schild G C
J Gen Virol. 1980 Jun;48(Pt 2):383-9. doi: 10.1099/0022-1317-48-2-383.
Seventeen of twenty-six influenza A virus isolates of the H1N1 antigenic subtype and two of eleven H3N2 virus isolates from the 1977-78 season exhibited a ts phenotype, were restricted in plaquing in MDCK cells at 38.5 degrees C compared to 34 degrees C and appeared to be naturally occurring ts mutants. The cut-off temperature for two such ts H1N1 virus isolates was established as 38 degrees C. The ts viruses were as thermostable as non-ts isolates and no complementation was detected between the twelve ts viruses tested. Cloning studies with an H1N1 virus isolate with minimal ts properties indicated the presence of a mixed population of ts+ and ts particles. Analysis of seven recombinants of A/HK/117/77(N1N1) virus indicated that the ts lesion(s) was not located in the NA or HA proteins.
1977 - 1978年季节分离出的26株H1N1抗原亚型甲型流感病毒中有17株以及11株H3N2病毒中有2株表现出温度敏感（ts）表型，与34℃相比，在38.5℃时在MDCK细胞中的空斑形成受到限制，似乎是自然发生的ts突变体。确定了两株这样的ts H1N1病毒分离株的截止温度为38℃。ts病毒与非ts分离株一样耐热，在所测试的12株ts病毒之间未检测到互补作用。对具有最小ts特性的一株H1N1病毒分离株进行的克隆研究表明存在ts +和ts颗粒的混合群体。对A/HK/117/77（N1N1）病毒的七个重组体的分析表明，ts损伤不在NA或HA蛋白中。
