Chu C M, Wei B Y, Tian S F, Ren G F
Institute of Virology, Beijing, People's Republic of China.
Virus Res. 1992 Mar;22(3):207-14. doi: 10.1016/0168-1702(92)90052-b.
Naturally occurring temperature-sensitive (ts) strains have been found in large number in human influenza A viruses of all subtypes (J. Virol. 41 (1982) 353). Further studies have demonstrated that the ts phenotype of these viruses is host-dependent in that they are highly ts in chick embryos and chick embryonic cells, but are ts+ in MDCK cells. Previous studies have located by complementation tests the ts lesion of two H3N2 viruses (HK/8/68 and Ningxia/01/72, also known as Xia-ts) on the NP gene and that of two H1N1 viruses (Tianjin/78/77 and Beijing/1/79) on the M protein gene. By recombination and polyacrylamide electrophoresis migration of the RNA segments of its ts+ recombinant with PR8, the ts lesion of a later H3N2 virus A/Qi/39/79 has now been located on the M protein gene. The possibility for Qi/39/79 of acquiring the M gene lesion by reassortment with concurrently circulating Tianjin/78/77-like (H1N1) virus which also has ts lesion on the M gene was investigated. In contrast to Tianjin/78/77 (H1N1), however, Qi/39/79 complemented well with ts 51, a WSN ts strain with a single M gene lesion. Qi/39/79 and Tianjin/78/77 also complemented each other. Thus, there are two intra-segmental complementation groups of the M gene: Qi/39/79 belongs to one complementation group, while WSN ts 51 and Tianjin/78/77 belong to another. At present, there is no evidence of reassortment involving the genes concerned in the ts lesions of H3N2 and H1N1 viruses under natural conditions.
在所有亚型的人类甲型流感病毒中均发现了大量自然产生的温度敏感（ts）毒株（《病毒学杂志》41卷（1982年）第353页）。进一步研究表明，这些病毒的ts表型具有宿主依赖性，即它们在鸡胚和鸡胚细胞中是高度温度敏感的，但在MDCK细胞中是温度敏感阳性（ts+）。先前的研究通过互补试验确定了两种H3N2病毒（香港/8/68和宁夏/01/72，也称为夏-ts）的ts损伤位于核蛋白（NP）基因上，以及两种H1N1病毒（天津/78/77和北京/1/79）的ts损伤位于基质蛋白（M）基因上。通过将其ts+重组体与PR8的RNA片段进行重组和聚丙烯酰胺电泳迁移，现已确定后来的一种H3N2病毒A/齐/39/79的ts损伤位于M蛋白基因上。研究了A/齐/39/79与同时流行的天津/78/77样（H1N1）病毒通过基因重配获得M基因损伤的可能性，该病毒在M基因上也有ts损伤。然而，与天津/78/77（H1N1）不同，A/齐/39/79与ts 51（一种具有单个M基因损伤的WSN ts毒株）互补良好。A/齐/39/79和天津/78/77也相互互补。因此，M基因存在两个片段内互补组：A/齐/39/79属于一个互补组，而WSN ts 51和天津/78/77属于另一个互补组。目前，没有证据表明在自然条件下H3N2和H1N1病毒的ts损伤相关基因发生了重配。
