Norton D S, Heaton F W
J Inorg Biochem. 1980 Aug;13(1):1-9. doi: 10.1016/s0162-0134(00)80208-1.
Supernatant preparations from the liver, small intestine, and skeletal muscle of normal rats were separated on Sephadex G-150 into six Cu and Zn containing protein fractions with molecular weights of about 250,000, 153,000, 73,000, 41,000, 22,000, and 10,000. Small differences in molecular weight and metal binding were observed between tissues, but the corresponding fractions from all three tissues were generally very similar. Age affected the distribution of both metals, particularly in liver; growth from the weanling to adult stage was accompanied by the transfer of considerable amounts of Cu and Zn from the 10,000 to the 22,000 fraction. A small influence of sex on the distribution was found in skeletal muscle. After injection of 65Zn radioactivity initially entered the 10,000 fraction in liver; it was then transferred to the region of the 73,000 fraction and entered the 22,000 fraction most slowly. The metabolic importance and probable nature of the various fractions is discussed.
将正常大鼠肝脏、小肠和骨骼肌的上清液制剂在葡聚糖凝胶G - 150上进行分离，得到六个含铜和锌的蛋白质组分，其分子量分别约为250,000、153,000、73,000、41,000、22,000和10,000。各组织之间在分子量和金属结合方面观察到微小差异，但来自所有三个组织的相应组分通常非常相似。年龄影响两种金属的分布，尤其是在肝脏中；从断奶期到成年期的生长过程伴随着大量铜和锌从10,000组分转移到22,000组分。在骨骼肌中发现性别对分布有微小影响。注射65Zn后，放射性最初进入肝脏的10,000组分；然后转移到73,000组分区域，进入22,000组分的速度最慢。讨论了各组分的代谢重要性和可能的性质。
