van Amelsvoort J M, Sips H J, Apitule M E, van Dam K
Biochim Biophys Acta. 1980 Aug 14;600(3):950-60. doi: 10.1016/0005-2736(80)90497-6.
The localization of the sodium-dependent alanine uptake activity in rat liver cells was studied. Fractions representative of the canalicular, the contiguous (lateral) and the blood-sinusoidal surface of the hepatocyte were isolated by means of centrifugal fractionation and density gradient centrifugation. The distribution of various marker-enzyme activities in conjunction with the occurrence of alanine transport activity was studied both in fractions obtained after zonal density gradient centrifugation, and in the subcellular fractions mentioned above. It is concluded that the sodium-dependent alanine transport activity is primarily located in the blood-sinusoidal plasma membrane of the hepatocyte.
研究了大鼠肝细胞中钠依赖性丙氨酸摄取活性的定位。通过离心分级分离和密度梯度离心法分离出代表胆小管、相邻（侧面）和肝血窦表面的肝细胞组分。结合丙氨酸转运活性的出现，研究了区域密度梯度离心后获得的组分以及上述亚细胞组分中各种标记酶活性的分布。得出的结论是，钠依赖性丙氨酸转运活性主要位于肝细胞的肝血窦质膜中。
