含脂质噬菌体phi 6的结构。经Triton X-100处理后的破坏情况。

Structure of the lipid-containing bacteriophage phi 6. Disruption by Triton X-100 treatment.

作者信息

Bamford D H, Palva E T

出版信息

Biochim Biophys Acta. 1980 Sep 18;601(2):245-59. doi: 10.1016/0005-2736(80)90530-1.

Abstract

The structure of the lipid-containing bacteriophage phi 6 was studied by means of controlled Triton X-100 disruption and subsequent isolation of subviral particles. Rate-zonal centrifugation yielded two fractions, a nucleocapsid fraction with RNA, proteins P1, P2, P4, P7, P8, and about half of the protein P5 and a membrane fraction with associated proteins P3, P6, P9, P10, and the rest of the protein P5. Following isopycnic sucrose gradient centrifugation, an empty capsid fraction was obtained which lacked RNA but contained a protein composition similar to the nucleocapsid except for the absence of P5. The membrane fraction isolated after isopycnic centrifugation was morphologically indistinguishable from that isolated after rate-zonal centrifugation but contained only proteins P3, P6, P9 and P10. By treating phi 6 with Triton X-100 prior to isopycnic sucrose gradient centrifugation the viral membrane was further separated into submembrane structures and the attachment protein, P3, could be isolated in rather pure form.

摘要

通过可控的 Triton X - 100 裂解及随后亚病毒颗粒的分离，对含脂质噬菌体 phi 6 的结构进行了研究。速率区带离心产生了两个组分，一个是核衣壳组分，含有 RNA、蛋白质 P1、P2、P4、P7、P8 以及大约一半的蛋白质 P5；另一个是膜组分，含有相关蛋白质 P3、P6、P9、P10 以及其余的蛋白质 P5。等密度蔗糖梯度离心后，得到了一个空衣壳组分，该组分不含 RNA，但除了没有 P5 外，其蛋白质组成与核衣壳相似。等密度离心后分离得到的膜组分在形态上与速率区带离心后分离得到的膜组分没有区别，但只含有蛋白质 P3、P6、P9 和 P10。在等密度蔗糖梯度离心之前用 Triton X - 100 处理 phi 6，病毒膜进一步分离成亚膜结构，附着蛋白 P3 可以以相当纯的形式分离出来。