Allred C D, Margetts J, Hill H R
Biochim Biophys Acta. 1980 Aug 13;631(2):380-5. doi: 10.1016/0304-4165(80)90311-6.
Out studies suggest that luminol directly enhances the chemiluminescence of human neutrophils. We show that a significant peak in chemiluminescence production in a particle-free system occurs between 5 and 15 min following exposure of cells to micromolar concentrations of luminol. The response is directly related to dose over a wide rane of luminol concentrations and can be inhibited by superoxide dismutase (90%), catalase (100%) and sodium azide (40%). Evidence is presented which suggests that the effect of luminol eliciting a peak in neutrophil chemiluminescence is mediated within intact cells rather than at the cell membrane. Luminol may produce a peak in chemiluminescence by stimulating very low levels of hexose monophosphate shunt activity and superoxide generation or it may simply amplify light production generated by the production of excited oxygen radicals resulting from surface interactions.
我们的研究表明，鲁米诺可直接增强人类中性粒细胞的化学发光。我们发现，在无颗粒系统中，当细胞暴露于微摩尔浓度的鲁米诺后，化学发光产生的显著峰值出现在5至15分钟之间。在很宽的鲁米诺浓度范围内，该反应与剂量直接相关，并且可被超氧化物歧化酶（90%）、过氧化氢酶（100%）和叠氮化钠（40%）抑制。有证据表明，鲁米诺引发中性粒细胞化学发光峰值的作用是在完整细胞内介导的，而非在细胞膜上。鲁米诺可能通过刺激极低水平的己糖磷酸支路活性和超氧化物生成来产生化学发光峰值，或者它可能只是放大了由表面相互作用产生的激发氧自由基所产生的光。
