Verbeeck R K, James R C, Taber D F, Sweetman B J, Wilkinson G R
Biomed Mass Spectrom. 1980 Feb;7(2):58-60. doi: 10.1002/bms.1200070204.
A gas chromatographic mass spectrometric assay using selected ion monitoring for meperidine and normeperidine in blood and plasma is described. Both unlabeled and [3H]-phenyl ring labeled drugs may be determined simultaneously using [2H10] analogs as internal standards. Calibration curves are linear over the range 5--100 ng ml-1, the results reproducible (coefficient of variation < 5%), the sensitivity limit is about 5 ng using a 1 ml sample and no interference is present in blank blood or plasma, or by isotope inpurity. The method is shown to be applicable to measuring the concentration of meperidine and its metabolite, normeperidine, in blood, following simultaneous administration by the intravenous and oral routes of 25 mg each of unlabeled and [2H5] labeled drug, respectively.
本文描述了一种利用选择离子监测技术测定血液和血浆中哌替啶及去甲哌替啶的气相色谱-质谱分析法。使用[2H10]类似物作为内标，可同时测定未标记的和[3H]苯环标记的药物。校准曲线在5-100 ng/ml范围内呈线性，结果具有可重复性（变异系数<5%），使用1 ml样品时灵敏度极限约为5 ng，空白血液或血浆以及同位素杂质均无干扰。该方法适用于分别经静脉和口服途径同时给予25 mg未标记的和[2H5]标记的药物后，测定血液中哌替啶及其代谢产物去甲哌替啶的浓度。
