来自短角杜父鱼（Myoxocephalus scorpius）的抗冻蛋白：分离与特性分析

Antifreeze proteins from the shorthorn sculpin, Myoxocephalus scorpius: isolation and characterization.

作者信息

Hew C L, Fletcher G L, Ananthanarayanan V S

出版信息

Can J Biochem. 1980 May;58(5):377-83. doi: 10.1139/o80-049.

Abstract

The antifreeze proteins (AFP) of the shorthorn sculpin, Myoxocephalus scorpius, were isolated and compared with the AFP of the winter flounder. The shorthorn sculpin was found to contain one major and one minor antifreeze protein with an approximate molecular weight of 10 000--11 000 in the winter. The major AFP was isolated and characterized. It had many characteristics in common with the flounder AFP studied in our laboratories. These characteristics included its seasonal appearance, size, amino acid composition, the abundance of alanine in the composition, the extent of antifreeze activity, and the nature of the secondary structure. It is suggested that both the sculpin and flounder AFP are structurally homologous and belong to the same type of polypeptide antifreeze.

摘要

对短角杜父鱼（Myoxocephalus scorpius）的抗冻蛋白（AFP）进行了分离，并与美洲拟鲽的抗冻蛋白进行了比较。研究发现，短角杜父鱼在冬季含有一种主要抗冻蛋白和一种次要抗冻蛋白，其分子量约为10,000 - 11,000。对主要的抗冻蛋白进行了分离和表征。它与我们实验室研究的鲽鱼抗冻蛋白有许多共同特征。这些特征包括其季节性出现、大小、氨基酸组成、组成中丙氨酸的含量、抗冻活性程度以及二级结构的性质。有人认为，杜父鱼和鲽鱼的抗冻蛋白在结构上是同源的，属于同一类型的多肽抗冻蛋白。