King W P, Kissinger P T
Clin Chem. 1980 Sep;26(10):1484-91.
We describe the use of electrogenerated reactants for continuous on-line reaction detection with thin-layer hydrodynamic amperometry. The reagent is introduced into the liquid-chromatographic column effluent at a constant rate by using controlled-current electrochemistry. After the effluent passes through a short reaction coil, the reagent concentration is monitored at the detector. Reaction of eluted compounds with bromine is signalled by changes in the current detected. The direct electrochemical control of the reagent concentration allows changes to be made, even during the course of obtaining a chromatogram. Depending on the specific reagent or reaction, the reagent is supplied either by addition of a second stream or by direct generation in the mobile phase. The latter configuration provides sufficient baseline stability to permit detection of the uptake of as little as 10 pmol of reagent bromine. The technique has been used to detect nanograms of underivatized fatty acids, prostaglandins, and phenols after separation by reversed-phase liquid chromatography.
我们描述了利用电生反应物，通过薄层流体动力学安培法进行连续在线反应检测。使用控制电流电化学方法，将试剂以恒定速率引入液相色谱柱流出物中。流出物通过一个短反应盘管后，在检测器处监测试剂浓度。洗脱化合物与溴的反应通过检测到的电流变化发出信号。试剂浓度的直接电化学控制使得即使在获取色谱图的过程中也能够进行更改。根据特定的试剂或反应，试剂可以通过添加第二股流或在流动相中直接生成来供应。后一种配置提供了足够的基线稳定性，能够检测到低至10皮摩尔试剂溴的摄取量。该技术已用于在反相液相色谱分离后检测纳克级未衍生化的脂肪酸、前列腺素和酚类。
