Steinick L E, Wieslander A, Johansson K E, Liss A
J Bacteriol. 1980 Sep;143(3):1200-7. doi: 10.1128/jb.143.3.1200-1207.1980.
The membrane composition of 11 strains of Acholeplasma laidlawii, including three strains persistently infected with mycoplasmaviruses MVL51, MVL2, and MVL3, was studied and correlated with mycoplasmavirus sensitivity. Membranes of the strains had similiar sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns, and all strains were inhibited by an antiserum produced against membranes from one of the strains. The amounts of integral membrane proteins solubilized by the nonionic detergent Tween 20 differed considerably. Therefore, characteristic crossed immunoelectrophoresis patterns were obtained for each strain. Strains persistently infected with MVL2 and MVL3 were notably different from the noninfected host. The ability to propagate any of the viruses was not correlated with sodium dodecyl sulfate-polyacrylamide gel electrophoresis or crossed immunoelectrophoresis patterns. The persistently infected strains had a characteristic lipid composition. MVL51-resistant strains, including a resistant clone selected from a sensitive strain, were characterized by a large monoglucosyldiglyceride/diglucosyldiglyceride ratio and trace amounts of diphosphatidylglyceol (as opposed to the sensitive strains). Differences in lipid composition in A. laidlawii seem to affect the relationship between cells and viruses.
对11株莱氏无胆甾原体（Acholeplasma laidlawii）的膜组成进行了研究，其中包括三株持续感染支原体病毒MVL51、MVL2和MVL3的菌株，并将其与支原体病毒敏感性相关联。这些菌株的膜具有相似的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳图谱，并且所有菌株都被针对其中一个菌株的膜产生的抗血清所抑制。非离子去污剂吐温20溶解的整合膜蛋白的量有很大差异。因此，每个菌株都获得了特征性的交叉免疫电泳图谱。持续感染MVL2和MVL3的菌株与未感染的宿主明显不同。任何一种病毒的繁殖能力与十二烷基硫酸钠-聚丙烯酰胺凝胶电泳或交叉免疫电泳图谱均无关联。持续感染的菌株具有特征性的脂质组成。对MVL51具有抗性的菌株，包括从敏感菌株中选出的一个抗性克隆，其特征是单葡萄糖二甘油酯/双葡萄糖二甘油酯比例大且二磷脂酰甘油含量微量（与敏感菌株相反）。莱氏无胆甾原体脂质组成的差异似乎会影响细胞与病毒之间的关系。
