Glew W B, Roberts W P, Malinin G I, Nigra T P
J Invest Dermatol. 1980 Sep;75(3):230-4. doi: 10.1111/1523-1747.ep12523233.
Photoactive 8-methoxypsoralen (8-MOP) levels in human and animal sera were determined by bioassay with Staphylcoccus aureus serving as the test organism. 8-MOP was extracted from sera with as 9:1 (V/V) ethyl acetate-n-hexane mixture and reconstituted in an aqueous medium following evaporation of the extractant. Bacterial suspensions containing extracted 8-MOP were irradiated for predetermined intervals with longwave ultraviolet light (UVA) at an intensity of 2.1 mw/cm2. Cultures containing known amounts of 8-MOP were used as standards and cytotoxicity (i.e., drug levels) determined by colony counts. The detection limit for 8-MOP was 5 ng/ml with an accuracy of +/- 10% above 10 ng/ml. Concomitant determinations of 8-MOP levels by high pressure liquid chromatography (HPLC) were in excellent agreement with the bioassay results.
采用金黄色葡萄球菌作为测试生物，通过生物测定法测定人和动物血清中的光活性8-甲氧基补骨脂素（8-MOP）水平。用9:1（V/V）乙酸乙酯-正己烷混合物从血清中提取8-MOP，提取物蒸发后在水性介质中复溶。含有提取的8-MOP的细菌悬液用强度为2.1 mw/cm2的长波紫外线（UVA）照射预定时间间隔。含有已知量8-MOP的培养物用作标准品，通过菌落计数确定细胞毒性（即药物水平）。8-MOP的检测限为5 ng/ml，在10 ng/ml以上时准确度为±10%。通过高压液相色谱法（HPLC）同时测定8-MOP水平与生物测定结果非常一致。
