人脐血淋巴细胞的体外初次抗体应答。

Primary in vitro antibody response in human cord blood lymphocytes.

作者信息

Pascal C, Galanaud P, Dormont J, Wallon C

出版信息

J Reprod Immunol. 1980 Feb;1(5-6):275-83. doi: 10.1016/0165-0378(80)90001-7.

Abstract

Human cord blood lymphocytes were stimulated with trinitrophenyl--polyacrylamide beads (TNP--PAA) in order to induce a primary IgM anti-TNP response. With few exceptions, no anti-TPN response was obtained, whereas peripheral blood lymphomonocytic cells (PBL) from 18-mth-old children were able to respond to TNP-PAA. The addition of lipopolysaccharide (LPS) or of mitomycin-treated adult PBL to cultures of cord blood lymphocytes significantly enhanced their anti-TNP response, thus showing that functional anti-TNP B cell precursors are present in the human neonate.

摘要

用人脐血淋巴细胞与三硝基苯基 - 聚丙烯酰胺珠（TNP - PAA）刺激，以诱导原发性IgM抗TNP反应。除少数例外，未获得抗TPN反应，而18个月大儿童的外周血淋巴细胞（PBL）能够对TNP - PAA作出反应。将脂多糖（LPS）或丝裂霉素处理的成人PBL添加到脐血淋巴细胞培养物中，可显著增强其抗TNP反应，从而表明人类新生儿中存在功能性抗TNP B细胞前体。