人类体外抗体反应的年龄相关损伤。
Age-related impairment of the in vitro antibody response in the human.
作者信息
Delfraissy J F, Galanaud P, Dormont J, Wallon C
出版信息
Clin Exp Immunol. 1980 Jan;39(1):208-14.
We have used trinitrophenyl polyacrylamide beads (TNP-PAA) to induce a primary in vitro antibody response toward TNP in cultures of peripheral blood lymphocytes (PBL) from aged individuals. The response was virtually non-existent whereas PBL from young control individuals were able to respond. Recombination experiments showed that: (1) aged PBL did not suppress the response of young PBL; (2) young T cells enhanced the response of aged (unfractionated or T-depleted) cells; (3) aged T cells were unable to restore the response of young T-depleted cells, in contrast to young allogeneic T cells. Upon stimulation with Concanavalin A (Con A) aged PBL displayed a moderately diminished proliferative response and a normal ability to suppress the anti-TNP response of autologous PBL (in the few responders). However, contrasting with young PBL they could not suppress the anti-TNP response of young allogeneic PBL.
我们使用三硝基苯基聚丙烯酰胺珠（TNP-PAA）来诱导老年个体外周血淋巴细胞（PBL）培养物中针对TNP的原发性体外抗体反应。老年个体的反应几乎不存在，而来自年轻对照个体的PBL能够产生反应。重组实验表明：（1）老年PBL不会抑制年轻PBL的反应；（2）年轻T细胞增强了老年（未分离或T细胞耗尽）细胞的反应；（3）与年轻的同种异体T细胞相比，老年T细胞无法恢复年轻T细胞耗尽细胞的反应。在用伴刀豆球蛋白A（Con A）刺激后，老年PBL表现出增殖反应适度减弱，并且具有抑制自体PBL（少数应答者）抗TNP反应的正常能力。然而，与年轻PBL不同的是，它们不能抑制年轻同种异体PBL的抗TNP反应。
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