Enjalbert A, Arancibia S, Ruberg M, Priam M, Bluet-Pajot M T, Rotsztejn W H, Kordon C
Neuroendocrinology. 1980 Sep;31(3):200-4. doi: 10.1159/000123074.
VIP stimulated prolactin secretion from incubated rat hemipituitaries. Under the same conditions, the secretion of GH, LH, FSH was not affected. The stimulation of prolactin was dose-dependent, with an apparent affinity of VIP of 10.9 +/- 3.1 nM and a maximal stimulation of 57.7 +/- 4.2%. Secretin, a structurally related peptide, was also active at higher concentrations whereas another partial analogue, glucagon, was ineffective. The effect of VIP was not blocked by alpha-flupentixol, a potent dopaminergic antagonist, at concentrations which antagonized the dopamine inhibition of prolactin secretion. Stimulation by VIP and TRH was additive. Neither Met-enkephalin nor naloxone interfered with the response to VIP. It thus seems that specific VIP receptors are present on pituitary prolactin cells. VIP, present in the mediobasal hypothalamus and detected in the hypothalamo-hypophyseal portal blood therefore is a good candidate as a physiological PRF.
血管活性肠肽（VIP）刺激孵育的大鼠半垂体分泌催乳素。在相同条件下，生长激素（GH）、促黄体生成素（LH）、促卵泡生成素（FSH）的分泌未受影响。催乳素的刺激呈剂量依赖性，VIP的表观亲和力为10.9±3.1 nM，最大刺激为57.7±4.2%。促胰液素是一种结构相关的肽，在较高浓度时也有活性，而另一种部分类似物胰高血糖素则无效。在拮抗多巴胺对催乳素分泌抑制作用的浓度下，强效多巴胺能拮抗剂α-氟哌噻吨未阻断VIP的作用。VIP和促甲状腺激素释放激素（TRH）的刺激作用具有相加性。甲硫氨酸脑啡肽和纳洛酮均未干扰对VIP的反应。因此，垂体催乳素细胞上似乎存在特异性VIP受体。存在于下丘脑正中基底部并在下丘脑-垂体门脉血中检测到的VIP因此是一种很好的生理性催乳素释放因子（PRF）候选物。
