Try K
Scand J Clin Lab Invest. 1980 Jun;40(4):305-9. doi: 10.3109/00365518009092648.
When performing the ALAT-determination as recommended by the Scandinavian Committee on Enzymes, turbidity will develop due to a low ionic strength of the buffer. At the end of the pre-incubation period, the turbidity may still be developing, increasing the absorbance and lowering the ALAT-activity by 0-5 U/l. Turbidity and the error introduced in the assay, can be eliminated by increasing the ionic strength of the buffer by addition of NaCl.
按照斯堪的纳维亚酶学委员会的建议进行丙氨酸氨基转移酶(ALAT)测定时,由于缓冲液的离子强度较低,会产生浑浊。在预孵育期结束时,浑浊可能仍在发展,从而增加吸光度并使ALAT活性降低0 - 5 U/l。通过添加氯化钠增加缓冲液的离子强度,可以消除浑浊以及测定中引入的误差。