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浊度作为L-丙氨酸转氨酶(ALAT)测定方法中误差的一个原因。

Turbidity as a cause of error in the L-alanine transferase (ALAT) method.

作者信息

Try K

出版信息

Scand J Clin Lab Invest. 1980 Jun;40(4):305-9. doi: 10.3109/00365518009092648.

DOI:10.3109/00365518009092648
PMID:7414249
Abstract

When performing the ALAT-determination as recommended by the Scandinavian Committee on Enzymes, turbidity will develop due to a low ionic strength of the buffer. At the end of the pre-incubation period, the turbidity may still be developing, increasing the absorbance and lowering the ALAT-activity by 0-5 U/l. Turbidity and the error introduced in the assay, can be eliminated by increasing the ionic strength of the buffer by addition of NaCl.

摘要

按照斯堪的纳维亚酶学委员会的建议进行丙氨酸氨基转移酶(ALAT)测定时,由于缓冲液的离子强度较低,会产生浑浊。在预孵育期结束时,浑浊可能仍在发展,从而增加吸光度并使ALAT活性降低0 - 5 U/l。通过添加氯化钠增加缓冲液的离子强度,可以消除浑浊以及测定中引入的误差。

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