一种通过带有火焰离子化检测的薄层色谱法定量人类血小板中脂质含量的新方法。

A new method to quantitate lipid contents in human platelets by thin layer chromatography with flame ionization detection.

作者信息

Hiramatsu K, Nozaki H, Arimori S

出版信息

Tokai J Exp Clin Med. 1980 Apr;5(2):177-86.

Abstract

Lipid contents in human platelets from 21 healthy adults were analysed using thin-layer chromatography (TLC) in combination with a flame ionization detector (FID). Samples of 0.3 microliter extracted from 10(8) platelets were chromatographed on quartz rods coated with silica gel (Chromarods), and calibrated with an FID using free cholesterol and phosphatidylcholine as standards. The weight percentage of neutral lipids in human platelets obtained in this study was 14.6% which consisted mainly of free cholesterol, while that of phosphatidylethanolamine was 24.9%, phosphatidylserine plus phosphatidylinositol 6.8%, phosphatidylcholine 35.2% and sphingomyelin 18.6%. Free cholesterol in 10(8) platelets was estimated as 7 micrograms and phospholipids as 46 micrograms from standard calibrations. The minimum amount detectable by this TLC-FID method was 0.05 micrograms of standard cholesterol acetate and 0.1 microgram of lipids from 10(8) human platelets. The reproducibility was quite satisfactory and the procedure could be performed quickly and simply.

摘要

采用薄层色谱法（TLC）结合火焰离子化检测器（FID），对21名健康成年人的人体血小板中的脂质成分进行了分析。从10⁸个血小板中提取的0.3微升样品在涂有硅胶的石英棒（Chromarods）上进行色谱分析，并以游离胆固醇和磷脂酰胆碱为标准品，用FID进行校准。本研究中获得的人体血小板中性脂质的重量百分比为14.6%，主要由游离胆固醇组成，而磷脂酰乙醇胺的重量百分比为24.9%，磷脂酰丝氨酸加磷脂酰肌醇为6.8%，磷脂酰胆碱为35.2%，鞘磷脂为18.6%。根据标准校准，10⁸个血小板中的游离胆固醇估计为7微克，磷脂为46微克。这种TLC-FID方法的最低检测量为0.05微克标准醋酸胆固醇和0.1微克来自10⁸个人体血小板的脂质。重现性相当令人满意，该程序可以快速简便地进行。