关于ATP与Mg2+、Ca2+及单胺相互作用的31P和1H核磁共振研究（作者译）

[31P- and 1H-NMR-investigations about the interaction of ATP with Mg2+, Ca2+, and monoamines (author's transl)].

作者信息

Sapper H, Gohl W, Lohmann W

出版信息

Z Naturforsch C Biosci. 1980 Jul-Aug;35(7-8):569-77.

Abstract

The interaction between aromatic amines and ATP is based upon an electrostatic attraction of the protonated amino groups to the negatively charged phosphate groups of ATP combined with a stacking association between the ring systems. The complexes formed are relatively weak (association constants 4--48 M-1), shortlived (life time smaller than 0.02 s), and mainly 1:1 in stoichiometry preferring the terminal phosphate of ATP as the site of association. The divalent metal ions Mg2+ and Ca2+ bond stronger than the amines (association constants 1500 and 100 M-1, resp.) prefer the beta-phosphate group changing thereby the geometry of the ATP phosphate chain. Thus, they reduce the amine-ATP association in ternary mixtures.

摘要

芳香胺与ATP之间的相互作用基于质子化氨基与ATP带负电荷的磷酸基团之间的静电吸引，以及环系统之间的堆积缔合。形成的复合物相对较弱（缔合常数为4 - 48 M⁻¹），寿命较短（小于0.02 s），化学计量比主要为1:1，倾向于以ATP的末端磷酸基团作为缔合位点。二价金属离子Mg²⁺和Ca²⁺的结合比胺更强（缔合常数分别为1500和100 M⁻¹），它们倾向于β - 磷酸基团，从而改变了ATP磷酸链的几何形状。因此，它们会降低三元混合物中胺与ATP的缔合。