[Incorporation of (32P)orthosphate into DNA of the mouse spleen under influence of antigen (author's transl)].

Antigens cause an increase of the DNA synthesis in the spleen of mice as shown by the incorporation of [32P]orthophosphate and [3H]thymidine. In the present paper, the incorporation of [32P]orthophosphate into the single deoxymononucletides has been studied under influence of the antigen bovine serum albumin. For this purpose, the labelled DNA was decomposed to the deoxymononucleotides and their specific activities determined. Using [32P]orthophosphate, it was found that the activity of the DNA increased continuously during an observation period of 8 h. While after one hour the relative specific activity of dTMP was highest and that of dAMP lowest, the relative specific activities of the deoxymononucleotides had become equal after 8 h. Under the influence of bovine serum albumin, the incorporation of [32P]orthophosphate increased in he main band DNA as well as in the satellite DNA. The antigen had no effect on the distribution of the specific activities of the deoxymononucleotides.