Uecker W, Kubsch D, Lutz D, Kröger H
Z Naturforsch C Biosci. 1980 Jul-Aug;35(7-8):599-604.
Antigens cause an increase of the DNA synthesis in the spleen of mice as shown by the incorporation of [32P]orthophosphate and [3H]thymidine. In the present paper, the incorporation of [32P]orthophosphate into the single deoxymononucletides has been studied under influence of the antigen bovine serum albumin. For this purpose, the labelled DNA was decomposed to the deoxymononucleotides and their specific activities determined. Using [32P]orthophosphate, it was found that the activity of the DNA increased continuously during an observation period of 8 h. While after one hour the relative specific activity of dTMP was highest and that of dAMP lowest, the relative specific activities of the deoxymononucleotides had become equal after 8 h. Under the influence of bovine serum albumin, the incorporation of [32P]orthophosphate increased in he main band DNA as well as in the satellite DNA. The antigen had no effect on the distribution of the specific activities of the deoxymononucleotides.
如通过[32P]正磷酸盐和[3H]胸腺嘧啶核苷的掺入所示，抗原会导致小鼠脾脏中DNA合成增加。在本文中，研究了在抗原牛血清白蛋白的影响下[32P]正磷酸盐掺入单个脱氧单核苷酸的情况。为此，将标记的DNA分解为脱氧单核苷酸并测定其比活性。使用[32P]正磷酸盐发现，在8小时的观察期内DNA活性持续增加。虽然1小时后dTMP的相对比活性最高而dAMP的最低，但8小时后脱氧单核苷酸的相对比活性变得相等。在牛血清白蛋白的影响下，[32P]正磷酸盐在主带DNA以及卫星DNA中的掺入均增加。该抗原对脱氧单核苷酸比活性的分布没有影响。
