Watanabe K
Ann Otol Rhinol Laryngol. 1980 May-Jun;89(3 Pt 1):241-8. doi: 10.1177/000348948008900311.
The activity of endogenous peroxidase was demonstrated in light ciliated cells, mature goblet cells, and nonciliated serous cells, but not in basal cells, intermediate cells, dark ciliated cells or immature goblet cells of the upper tracheal epithelium of rats, using the diaminobenzidine method for cytochemical demonstration of peroxidase activity. The peroxidase activity ws the most intense in the epithelium of the first tracheal ring and gradually diminished at lower tracheal levels, becoming extremely faint in the vicinity of the middle of the trachea. The activity was localized in cisternae of rough-surfaced endoplasmic reticulum (r-ER) including the nuclear envelope, some vesicles and saccules of the Golgi complex, secretory granules, and small apical vesicles. It is believed that the basal cells were transformed into the intermediate cells, and some of the intermediate cells were turned into the ciliated cells, while other intermediate cells were differentiated into the goblet cells. The dark ciliated cells proved to be the immature ciliated cells immediately after the transformation from the intermediate cells. In these cells, peroxidase is not yet produced.
采用二氨基联苯胺法对过氧化物酶活性进行细胞化学鉴定，结果显示，大鼠气管上段上皮的亮纤毛细胞、成熟杯状细胞和非纤毛浆液细胞内存在内源性过氧化物酶活性，而基底细胞、中间细胞、暗纤毛细胞或未成熟杯状细胞内则无此活性。过氧化物酶活性在气管第一环的上皮中最为强烈，在气管较低水平逐渐减弱，在气管中部附近变得极其微弱。该活性定位于粗面内质网（r-ER）的池，包括核膜、高尔基体复合体的一些小泡和扁平囊、分泌颗粒以及顶端小泡。据信，基底细胞转化为中间细胞，部分中间细胞转变为纤毛细胞，而其他中间细胞则分化为杯状细胞。暗纤毛细胞被证明是刚从中间细胞转化而来的未成熟纤毛细胞。在这些细胞中，尚未产生过氧化物酶。
