Scali V, Pellicciari C, Montanelli E, Bernocchi G
Basic Appl Histochem. 1980;24(2):135-44.
In the midgut epithelium of the walking stick insect Bacillus rossius, nuclei belong to two main cytological categories: nuclei with ordinary chromatin (ON) showing a polyploid series of Feulgen-DNA contents (2, 4, 8, 16C) and nuclei with DNA-RNA bodies (NWB) varying from 9 to 34C. Cells with ON are either small basal or large secretory elements, while those with NWB always belong to the second type. The Feulgen kinetics profiles obtained after formalin fixation, as well as their least squares fit by computer analysis, have shown that with increasing ploidy both the aldehydic group availability and the resistance to depolymerization increase in the genomes of ON; on the contrary, NWB have a depurination rate and resistance to depolymerization significantly lower than the ON. The kind and magnitude of the changes observed, while suggesting a gradually different DNA-protein relationship with increasing ploidy and concurrent maturation of ON, also indicate a deeper chromatin reorganization for NWB.
在竹节虫巴氏竹节虫（Bacillus rossius）的中肠上皮中，细胞核属于两种主要的细胞学类型：具有普通染色质的细胞核（ON）呈现出福尔根 - DNA含量的多倍体系列（2C、4C、8C、16C），以及具有DNA - RNA体的细胞核（NWB），其含量从9C到34C不等。具有ON的细胞要么是小的基底细胞，要么是大的分泌细胞，而具有NWB的细胞总是属于第二种类型。福尔马林固定后获得的福尔根动力学图谱，以及通过计算机分析进行的最小二乘法拟合表明，随着倍性增加，ON基因组中的醛基可用性和抗解聚能力均增加；相反，NWB的脱嘌呤率和抗解聚能力明显低于ON。观察到的变化的种类和程度，虽然表明随着倍性增加以及ON的同时成熟，DNA - 蛋白质关系逐渐不同，但也表明NWB存在更深层次的染色质重组。
