Liao M J, Prestegard J H
Biochim Biophys Acta. 1980 Oct 2;601(3):453-61. doi: 10.1016/0005-2736(80)90549-0.
Fusion of phosphatidylcholine-phosphatidic acid mixed lipid vesicles has been studied under the influence of the divalent Ca2+, Mg2+, Cd2+, and Ba2+, which range in size from much smaller to much larger, than Ca2+. Fusion has also been studied under the influence of Eu3+, which has a similar radius but different charge. The effects of these ions are reflected in the different degrees of fusion determined by changes in vesicle size, and in the varying fusion rates monitored by 1H-NMR spectroscopy. Cd2+, which has an ionic radius similar to Ca2+, exhibits the same effect on fusion as Ca2+, while other ions show lower efficiencies. This suggests that the vesicle fusion intermediate has a geometry ideally suited to the binding of Ca2+ or Cd2+.
在二价离子Ca2+、Mg2+、Cd2+和Ba2+的影响下，对磷脂酰胆碱 - 磷脂酸混合脂质囊泡的融合进行了研究，这些离子的大小与Ca2+相比范围从小得多到大多了。还在Eu3+影响下研究了融合，Eu3+具有相似的半径但电荷不同。这些离子的作用反映在由囊泡大小变化所确定的不同融合程度上，以及通过1H - NMR光谱监测的不同融合速率上。Cd2+的离子半径与Ca2+相似，对融合表现出与Ca2+相同的作用，而其他离子的效率较低。这表明囊泡融合中间体具有一种几何结构非常适合Ca2+或Cd2+的结合。
