蛋白酶抑制剂对大鼠各种组织中组氨酸脱羧酶活性的影响及酶的测定

The effects of protease inhibitors on histidine decarboxylase activities and assay of enzyme in various rat tissues.

作者信息

Yamada M, Watanabe T, Harino S, Fukui H, Wada H

出版信息

Biochim Biophys Acta. 1980 Oct;615(2):458-64. doi: 10.1016/0005-2744(80)90511-2.

Abstract

Histidine decarboxylase (L-histidine carboxy-lyase, EC 4.1.1.22) of an extract of rat stomach was inactivated by a pancreatic extract. This inactivation was prevented by the protease inhibitors leupeptin, antipan, chymostatin, pepstatin, Trasylol and phenylmethanesulfonyl fluoride. Leupeptin, antipain, chymostatin and pepstatin together and phenylmethanesulfonyl fluoride alone prevented complete inactivation of the enzyme, while Trasylol had a weak protective effect. The inactivation and protection of histidine decarboxylase purified from whole fetal rats were similar to those of the stomach enzyme: both enzymes were strongly inactivated by trypsin and chymotrypsin, but not by elatase or carboxypeptidase Y. The histidine decarboxylase activities of various rat tissues were assayed in the presence of protease inhibitors: activity was highest in mast cells followed by the whole bodies of fetal rats and the stomach, while the activities were lower in decreasing order in the brain, spleen, lung and liver. Heart and kidney had no activity.

摘要

大鼠胃提取物中的组氨酸脱羧酶（L-组氨酸羧基裂解酶，EC 4.1.1.22）被胰腺提取物灭活。蛋白酶抑制剂亮抑酶肽、抗蛋白酶、抑肽酶、胃蛋白酶抑制剂、抑肽酶和苯甲基磺酰氟可防止这种灭活。亮抑酶肽、抗蛋白酶、抑肽酶和胃蛋白酶抑制剂共同作用以及单独使用苯甲基磺酰氟可防止该酶完全失活，而抑肽酶的保护作用较弱。从整个胎鼠中纯化的组氨酸脱羧酶的失活和保护情况与胃酶相似：两种酶均被胰蛋白酶和糜蛋白酶强烈灭活，但不被弹性蛋白酶或羧肽酶Y灭活。在蛋白酶抑制剂存在的情况下测定了各种大鼠组织的组氨酸脱羧酶活性：肥大细胞中的活性最高，其次是胎鼠整体和胃，而脑、脾、肺和肝中的活性依次降低。心脏和肾脏无活性。