Aasen A O, Resch F, Semb B J, Dale J, Stadskleiv K, Lilleaasen P, Frøysaker T
Eur Surg Res. 1980;12(3):199-207. doi: 10.1159/000128125.
The present study was designed for long-term evaluation of the recently developed Hall-Kaster heart valve prosthesis. Mitral valve replacement was performed in 33 dogs. A detailed description of the operative procedure used is given. Extracorporeal circulation was instituted using a nonhemic priming volume resulting in an extreme hemodilution with hematocrit of about 15%. 19 of the animals died within 24 h, while 7 survived the observation period of 4--6 weeks. Thrombus on the valve was the major cause of early and late deaths. Anticoagulants were therefore given, and the therapy was made more extensive as the study progressed. The anticoagulation regime finally comprised warfarin administration preoperatively and throughout the experiment, and heparin infusion during the first 24 h postoperatively. Neomycin was used for preoperative sterilization of the gut. Later, cephalotin and penicillin were given. The use of these anticoagulants and antibiotics was found to be of major significance in providing us with a satisfactory canine model for long-term in vivo evaluation of the prosthesis.
本研究旨在对最近研发的霍尔-卡斯特心脏瓣膜假体进行长期评估。对33只犬实施了二尖瓣置换术。文中给出了所采用手术过程的详细描述。使用无血预充量建立体外循环，导致血细胞比容约为15%的极度血液稀释。19只动物在24小时内死亡，而7只存活至4 - 6周的观察期。瓣膜上的血栓是早期和晚期死亡的主要原因。因此给予抗凝剂，并且随着研究进展治疗更加广泛。抗凝方案最终包括术前及整个实验过程中给予华法林，术后最初24小时内输注肝素。新霉素用于术前肠道灭菌。后来给予头孢噻吩和青霉素。发现使用这些抗凝剂和抗生素对于为我们提供一个用于假体长期体内评估的满意犬模型具有重要意义。
