Ogino T
Gan. 1980 Jun;71(3):381-6.
Using a culture line of renal carcinoma of Syrian hamster (HKC-400), the distribution of DNA content in individual cells was analyzed by autoradiography after pulse and continuous labeling with 3H-thymidine in order to investigate the behavior of the non-proliferative cell compartment. Additional data used were the cell cycle parameters obtained by the fraction of labeled mitoses (FLM) method and the growth rate. The principle of analysis is to compare the cell fractions having G1 and G2 DNA contents with the fractions of G1 and G2 phase cells. It was concluded that even in the exponential phase of growth 31% of cells were blocked in either the G1 or G2 DNA region (Q2 = 22% and Q2 = 9%, and the cell loss rate from Q1 was higher than from Q2.
使用叙利亚仓鼠肾癌培养系（HKC - 400），通过用³H - 胸腺嘧啶脉冲标记和连续标记后进行放射自显影分析单个细胞中的DNA含量分布，以研究非增殖细胞区室的行为。使用的其他数据是通过标记有丝分裂分数（FLM）方法获得的细胞周期参数和生长速率。分析原理是将具有G1和G2 DNA含量的细胞分数与G1和G2期细胞分数进行比较。得出的结论是，即使在生长指数期，31%的细胞在G1或G2 DNA区域被阻滞（Q2 = 22%且Q2 = 9%），并且从Q1的细胞丢失率高于从Q2的细胞丢失率。
