Spindle A
In Vitro. 1980 Aug;16(8):669-74. doi: 10.1007/BF02619196.
Eagle's basal medium, modified to contain essential amino acids at the concentrations optimal for mouse blastocyst hatching, attachment, and outgrowth, supported in vitro development of the mouse blastocyst better than other tissue culture media tested. This medium was improved for growth and differentiation of the inner cell mass by doubling the concentration of amino acids and glucose and by adding uridine (10(-5) M) and beta-mercaptoethanol (10(-5) M). In this improved medium nearly all blastocysts grown from the two-cell stage hatched and formed trophoblast outgrowths, and 62% developed into two-layer egg cylinders.
伊格尔基础培养基经改良后含有对小鼠囊胚孵化、附着和生长最适宜浓度的必需氨基酸，在体外对小鼠囊胚发育的支持作用优于所测试的其他组织培养基。通过将氨基酸和葡萄糖的浓度加倍，并添加尿苷（10⁻⁵ M）和β-巯基乙醇（10⁻⁵ M），该培养基在促进内细胞团的生长和分化方面得到了改进。在这种改良培养基中，几乎所有从二细胞阶段开始培养的囊胚都孵化并形成了滋养层生长物，62%发育成了双层卵圆柱。
