Mullin J M, Weibel J, Diamond L, Kleinzeller A
J Cell Physiol. 1980 Sep;104(3):375-89. doi: 10.1002/jcp.1041040311.
Cells of confluent cultures of the established pig renal epithelial line, LLC-PK1, accumulate alpha-methyl-D-glucoside against a concentration gradient. This transport system is strongly inhibited by phlorizin and 6-deoxy-D-glucose, moderately inhibited by phloretin, and only weakly inhibited by 3-0-methyl-D-glucose, paralleling the situation in mammalian kidney. The time courses for the uptake of alpha-methyl-D-glucoside and for the carrier-mediated but passive uptake of 3-0-methyl-D-glucose are identical to those seen in mammalian kidney. Subconfluent cultures of LLC-PK1 cells are unable to accumulate alpha-methyl-D-glucoside, and their transport of this glucose analog is less sensitive to phlorizin inhibition than is the transport system in confluent cultures. Transmission electron micrographs show that cells from subconfluent cultures lack the microvillous surface seen in cells from confluent cultures. Cell density is thus a factor in the occurrence of structural and functional differentiated properties related to transport in these cells.
已建立的猪肾上皮细胞系LLC-PK1的汇合培养细胞能逆浓度梯度积累α-甲基-D-葡萄糖苷。该转运系统受到根皮苷和6-脱氧-D-葡萄糖的强烈抑制，受到根皮素的中度抑制，仅受到3-O-甲基-D-葡萄糖的微弱抑制，这与哺乳动物肾脏中的情况相似。α-甲基-D-葡萄糖苷摄取以及载体介导的但被动的3-O-甲基-D-葡萄糖摄取的时间进程与在哺乳动物肾脏中观察到的相同。LLC-PK1细胞的亚汇合培养物无法积累α-甲基-D-葡萄糖苷，并且它们对这种葡萄糖类似物的转运对根皮苷抑制的敏感性低于汇合培养物中的转运系统。透射电子显微镜照片显示，亚汇合培养物中的细胞缺乏汇合培养物中细胞所具有的微绒毛表面。因此，细胞密度是这些细胞中与转运相关的结构和功能分化特性出现的一个因素。
