高效液相色谱法同时测定血浆和尿液中保泰松及其代谢产物
Simultaneous determination of phenylbutazone and its metabolites in plasma and urine by high-performance liquid chromatography.
作者信息
Marunaka T, Shibata T, Minami Y, Umeno Y
出版信息
J Chromatogr. 1980 Sep 12;183(3):331-8. doi: 10.1016/s0378-4347(00)81713-2.
A high-performance liquid chromatographic method was developed for the simultaneous determination of phenylbutazone and its metabolites, oxyphenbutazone and gamma-hydroxy-phenylbutazone, in plasma and urine. Samples were acidified with hydrochloric acid and extracted with benzene--cyclohexane (1:1, v/v). The extract was redissolved in methanol and chromatographed on a muBondapak C18 column using a mobile phase of methanol--0.01 M sodium acetate buffer (pH 4.0) in a linear gradient (50 to 100% methanol at 5% min; flow-rate 2.0 ml/min) in a high-performance liquid chromatograph equipped with an ultraviolet absorbance detector (254 nm). The detection limit for phenylbutazone, oxyphenbutazone and for gamma-hydroxyphenylbutazone was 0.05 microgram/ml. A precise and sensitive assay for the determination of phenylbutazone and its metabolites was established.
建立了一种高效液相色谱法，用于同时测定血浆和尿液中的保泰松及其代谢物羟基保泰松和γ-羟基保泰松。样品用盐酸酸化，并用苯 - 环己烷（1:1，v/v）萃取。萃取物重新溶解于甲醇中，并在配备紫外吸收检测器（254nm）的高效液相色谱仪上，使用甲醇 - 0.01M醋酸钠缓冲液（pH 4.0）的流动相，以线性梯度（5%分钟内从50%到100%甲醇；流速2.0ml/min）在μBondapak C18柱上进行色谱分析。保泰松、羟基保泰松和γ-羟基保泰松的检测限为0.05微克/毫升。建立了一种用于测定保泰松及其代谢物的精确且灵敏的分析方法。
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