Kane E S, Barone L M
J Comp Neurol. 1980 Aug 15;192(4):797-826. doi: 10.1002/cne.901920412.
The normal population of neurons and their distributions within the dorsal nucleus of the lateral lemniscus were studied in both Nissl-stained celloidin and frozen sections and in Golgi impregnations from brains of mature cats. According to axial measurements of somata in Nissl-stained material, neurons of the dorsal nucleus of the lateral lemniscus (DNLL) were classified by width:length ratio (r) into round (0.80 less than or equal to r less than or equal to 1.0), ovoid (0.65 < r < 0.80), or elongate (r less than or equal to 0.65) types. These same neurons could also be classed by average diameter (Dm) as large (Dm greater than or equal to 22), medium (12.0 less than or equal to Dm < 22.0), or small (Dm < 12.0). A combination of data on ratios (shape) and average diameters (size) provided the following possible categories of Nissl-stained, DNLL neurons: large round (LR), large ovoid (LO), large elongate (LE), medium round (MR), medium ovoid (MO), medium elongate (ME), small round (SR), small ovoid (SQ), and small elongate (SE). Very few small cells were found, however. Quantitative studies of the distributions of cell type within the whole DNLL showed (1) most medium-sized and most LE cells in the caudal third of the DNLL and (2) most LO and LR cells dorsally located in the rostral third of the DNLL. There were progressively more large and more round types along the caudal-to-rostral axis. In Golgi impregnations of the DNLL, all medium and large cell types, but no small cell types (defined in the Nissl study) were found. Golgi material showed (1) subdivisions of the LO class into vertical (LOV) and horizontal (LOH) types, and (2) radiate (MRR) and oriented (MRO) subclasses of MR neurons according to dendritic arbor and cytology, orientation within the DNLL, and axonal morphology. Examples from all classes of large cells (particularly, LE cells) could have ventrally directed axons. These ventrally directed axons might be efferents to the cochlear nucleus, known from our previous work. A strong horizontal orientation of most DNLL cell somata and dendrites, shown in both our Nissl and Golgi material, is discussed in relation to known inputs to the DNLL. Correlations of our morphological findings with limited electrophysiological data on the DNLL are also discussed.
利用尼氏染色的火棉胶切片和冰冻切片以及成熟猫脑的高尔基浸染法，研究了外侧丘系背核内神经元的正常数量及其分布情况。根据尼氏染色材料中神经元胞体的轴向测量数据，外侧丘系背核（DNLL）的神经元按宽长比（r）分为圆形（0.80≤r≤1.0）、卵形（0.65＜r＜0.80）或细长形（r≤0.65）三种类型。这些神经元也可按平均直径（Dm）分为大（Dm≥22）、中（12.0≤Dm＜22.0）或小（Dm＜12.0）三种类型。综合比率（形状）和平均直径（大小）的数据，尼氏染色的DNLL神经元可能有以下几种类型：大圆形（LR）、大卵形（LO）、大细长形（LE）、中圆形（MR）、中卵形（MO）、中细长形（ME）、小圆形（SR）、小卵形（SQ）和小细长形（SE）。然而，发现的小细胞很少。对整个DNLL内细胞类型分布的定量研究表明：（1）DNLL尾侧三分之一区域内大多数中型和大多数LE细胞；（2）大多数LO和LR细胞位于DNLL头侧三分之一区域的背侧。沿着尾到头的轴线，大细胞和圆形细胞的类型逐渐增多。在DNLL的高尔基浸染标本中，发现了所有中型和大型细胞类型，但未发现小细胞类型（如尼氏研究中所定义）。高尔基材料显示：（1）LO类可细分为垂直型（LOV）和水平型（LOH）；（2）根据树突分支、细胞学特征、在DNLL内的方向以及轴突形态，MR神经元可分为辐射型（MRR）和定向型（MRO）亚类。所有大型细胞类型（特别是LE细胞）的例子都可能有腹侧方向的轴突。这些腹侧方向的轴突可能是传出纤维，通向耳蜗核，这在我们之前的研究中已得到证实。我们在尼氏和高尔基材料中均显示，大多数DNLL细胞胞体和树突具有强烈的水平方向，本文结合已知的DNLL输入对此进行了讨论。还讨论了我们的形态学发现与关于DNLL的有限电生理数据之间的相关性。
