人体血液白细胞的重力沉降分析
Gravity sedimentation analysis of human blood leukocytes.
作者信息
Catsimpoolas N, Skrabut E M, Griffith A L, Valeri C R
出版信息
J Biochem Biophys Methods. 1980 Mar;2(3):147-53. doi: 10.1016/0165-022x(80)90022-6.
A new method of sedimentation analysis of human blood leukocytes is described. Platelets, lymphocytes, monocytes, and polymorphonuclear cells isolated from normal human peripheral blood have been analyzed alone and in mixture by gravity sedimentation, employing a computerized scanning instrument. All four classes could be clearly resolved from each other exhibiting sedimentation velocities of 0.06 +/- 0.00, 1.04 +/- 0.11, 1.27 +/- 0.15 and 1.89 +/- 0.21 x 10(-4) cm/s, respectively, at 37 degrees C in a 2.5--6.25% Ficoll gradient in Medium 199. Less than 10(6) cells can be used for analysis. Possible applications of the method are discussed.
本文描述了一种人类血液白细胞沉降分析的新方法。从正常人外周血中分离出的血小板、淋巴细胞、单核细胞和多形核细胞，已通过重力沉降法单独或混合进行分析，采用的是计算机化扫描仪器。在37摄氏度下，于含2.5%-6.25%菲可的199培养基梯度中，所有这四类细胞彼此都能清晰分辨，其沉降速度分别为0.06±0.00、1.04±0.11、1.27±0.15和1.89±0.21×10⁻⁴厘米/秒。分析所用细胞数量可少于10⁶个。文中还讨论了该方法可能的应用。
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