Matheson D F, Oei R, Roots B I
Neurochem Res. 1980 Jul;5(7):683-95. doi: 10.1007/BF00964707.
The glycerophospholipid (GPL) content and acyl group compositions of isolated brain endothelial fractions have been determined in the developing rat. During development there is a marked change in the proportions of ethanolamine glycerophospholipids (EGP) to choline glycerophospholipids (CGP), the former rising while CGP falls with age. The acyl group compositions of plasmenylethanolamine (P-GPE) and 1,2-diacyl-sn-glycero-3-phosphocholine (D-GPC) after significantly during development; both show a decline in saturated fatty acids (SFAs) and a rise in the n-6/SFA ratio, in contrast to a constancy in composition of 1,2-diacyl-sn-glycero-3-phosphoethanolamine (D-GPE). The degree of change in the acyl group composition in a particular GPL fraction is related to the rate of its accumulation and to the proportional increase in concentration, fractions accumulating most rapidly or increasing markedly in concentration showing the greatest acyl group compositional change. The possible significance of the high proportion of SFAs in P-GPE and D-GPC fractions in the developing brain endothelial fraction is discussed in relation to the altering blood-brain barrier capacities observed with age.
已测定发育中大鼠分离的脑内皮部分的甘油磷脂(GPL)含量和酰基组成。在发育过程中,乙醇胺甘油磷脂(EGP)与胆碱甘油磷脂(CGP)的比例有显著变化,前者随年龄增长而上升,而CGP则下降。发育过程中,缩醛磷脂酰乙醇胺(P-GPE)和1,2-二酰基-sn-甘油-3-磷酸胆碱(D-GPC)的酰基组成有显著变化;与1,2-二酰基-sn-甘油-3-磷酸乙醇胺(D-GPE)组成恒定相反,二者均显示饱和脂肪酸(SFA)含量下降,n-6/SFA比值上升。特定GPL部分的酰基组成变化程度与其积累速率和浓度的比例增加有关,积累最快或浓度显著增加的部分显示出最大的酰基组成变化。结合随年龄观察到的血脑屏障能力变化,讨论了发育中脑内皮部分P-GPE和D-GPC部分中高比例SFA的可能意义。