培养的人内皮细胞的磷脂组成。
Phospholipid composition of cultured human endothelial cells.
作者信息
Murphy E J, Joseph L, Stephens R, Horrocks L A
机构信息
Department of Medical Biochemistry, Ohio State University, Columbus 43210.
出版信息
Lipids. 1992 Feb;27(2):150-3. doi: 10.1007/BF02535816.
Detailed analyses of the phospholipid compositions of cultured human endothelial cells are reported here. No significant differences were found between the phospholipid compositions of cells from human artery, saphenous and umbilical vein. However, due to the small sample sizes, relatively large standard deviations for some of the phospholipid classes were observed. A representative composition of endothelial cells is: phosphatidylcholine 36.6%, choline plasmalogen 3.7%, phosphatidylethanolamine 10.2%, ethanolamine plasmalogen 7.6%, sphingomyelin 10.8%, phosphatidylserine 7.1%, lysophosphatidylcholine 7.5%, phosphatidylinositol 3.1%, lysophosphatidylethanolamine 3.6%, phosphatidylinositol 4,5-bisphosphate 1.8%, phosphatidic acid 1.9%, phosphatidylinositol 4-phosphate 1.5%, and cardiolipin 1.9%. The cells possess high choline plasmalogen and lysophosphatidylethanolamine contents. The other phospholipids are within the normal biological ranges expected. Phospholipids were separated by high-performance liquid chromatography and quantified by lipid phosphorus assay.
本文报道了对培养的人内皮细胞磷脂组成的详细分析。在来自人动脉、大隐静脉和脐静脉的细胞磷脂组成之间未发现显著差异。然而，由于样本量较小，观察到某些磷脂类别的标准差相对较大。内皮细胞的代表性组成如下：磷脂酰胆碱36.6%、胆碱缩醛磷脂3.7%、磷脂酰乙醇胺10.2%、乙醇胺缩醛磷脂7.6%、鞘磷脂10.8%、磷脂酰丝氨酸7.1%、溶血磷脂酰胆碱7.5%、磷脂酰肌醇3.1%、溶血磷脂酰乙醇胺3.6%、磷脂酰肌醇4,5-二磷酸1.8%、磷脂酸1.9%、磷脂酰肌醇4-磷酸1.5%和心磷脂1.9%。这些细胞具有较高的胆碱缩醛磷脂和溶血磷脂酰乙醇胺含量。其他磷脂在预期的正常生物学范围内。磷脂通过高效液相色谱法分离，并通过脂质磷测定法定量。
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