人脑脊液中甲硫氨酸脑啡肽样物质的放射受体测定

Radioreceptor assay of methionine-enkephalin-like substance in human cerebrospinal fluid.

作者信息

Furui Tomoo, Kageyama Naoki, Haga Tatsuya, Ichiyama Arata, Fukushima Masanori

机构信息

Department of Neurosurgery, Nagoya University School of Medicine, Nagoya Japan Department of Biochemistry, Hamamatsu University School of Medicine, Hamamatsu Japan Department of Internal Medicine, Aichi Cancer Center, Nagoya Japan.

出版信息

Pain. 1980 Aug;9(1):63-72. doi: 10.1016/0304-3959(80)90029-9.

DOI:10.1016/0304-3959(80)90029-9

PMID:7422337

Abstract

A sensitive and simple radioreceptor assay system for measuring methionine(met)-enkephalin-like substance in human cerebrospinal fluid (CSF) was developed using a particulate fraction of rat brain as a receptor preparation and [3H]dihydromorphine as a radiolabeled ligand in the presence of 1 mM ethylenediamine tetracetate (EDTA) and 2 mM magnesium acetate. Metenkephalin-like substance was purified from CSF by the combination of Sephadex G-10 and SP-Sephadex (H+) column chromatographies to be free of sodium and large molecular weight substance such as beta-endorphin. The assays were carried out on samples obtained from normal subjects and patients with the disease of the brain or pituitary by lumbar or ventricular puncture. Mean level in samples obtained from normal subjects by lumbar puncture was 2.6 +/- 1.0 pmoles/ml.

摘要

利用大鼠脑的微粒部分作为受体制剂，以[3H]二氢吗啡作为放射性标记配体，在1 mM乙二胺四乙酸（EDTA）和2 mM乙酸镁存在的条件下，开发了一种灵敏且简单的放射受体分析系统，用于测量人脑脊液（CSF）中蛋氨酸（met）-脑啡肽样物质。通过葡聚糖凝胶G-10和SP-葡聚糖凝胶（H+）柱色谱相结合的方法，从脑脊液中纯化出蛋氨酸脑啡肽样物质，使其不含钠和β-内啡肽等大分子物质。对通过腰椎穿刺或脑室穿刺从正常受试者以及患有脑部或垂体疾病的患者中获取的样本进行检测。通过腰椎穿刺从正常受试者获得的样本中的平均水平为2.6 +/- 1.0皮摩尔/毫升。