通过高效液相色谱法将花生四烯酸与人和兔血小板孵育后的代谢产物完全分离。
Complete separation by high performance liquid chromatography of metabolites of arachidonic acid from incubation with human and rabbit platelets.
作者信息
VanRollins M, Ho S H, Greenwald J E, Alexander M, Dorman N J, Wong L K, Horrocks L A
出版信息
Prostaglandins. 1980 Sep;20(3):571-7. doi: 10.1016/0090-6980(80)90044-1.
Separation of all major cyclooxygenase and lipoxygenase metabolites of arachidonic acid were obtained by high performance liquid chromatography (HPLC). A C18 reverse-phase column was used in ion suppression mode to separate underivatized metabolites of arachidonic acid isolated from human and rabbit platelets. The metabolites were monitored by measuring radioactivity or ultraviolet light absorption at 192 nm (absorption by double bonds). Comparisons of TLC and HPLC separations demonstrated that the HPLC separation of metabolites of [1-14C]arachidonic acid was quantitative. HPLC also resolved several metabolites that were not detected by scanning of TLC separations.
通过高效液相色谱法（HPLC）分离了花生四烯酸的所有主要环氧化酶和脂氧化酶代谢产物。使用C18反相柱，以离子抑制模式分离从人和兔血小板中分离出的未衍生化花生四烯酸代谢产物。通过测量放射性或192nm处的紫外光吸收（双键吸收）来监测代谢产物。薄层色谱法（TLC）和HPLC分离的比较表明，[1-14C]花生四烯酸代谢产物的HPLC分离是定量的。HPLC还分离出了几种通过TLC分离扫描未检测到的代谢产物。
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